摘要
以５－溴－４－氯－３－吲哚磷酸盐和氯化硝基四氮唑蓝为底物，建立ＡＬＰ活力比色测定法。线性下限０．２Ｕ／Ｌ（皮－劳法）；两份标本ＣＶ值批内为１．０７％和１．５９％，批间为４．１３％和４．３８％；与皮－劳法（连续监测法）结果比较，相关系数为０．９９２。结果表明，此法简便、可靠、灵敏。
A colorimetric method for measuring alkaline phosphatase (ALP)
activity with
BCIP and NBT as substrates was established. The low limit of detection is 0 2 U/L (Bessy
Lowry
method). The within run CVs of two levels of ALP activity (5 0、1 0 U/L) are 1 07% and 1 59%,
between run CVs 4 13% and 4 38%, respectively. The correlation coefficient between this
method
and Bessy Lowry method (kinetic) is 0 992. The results showed that this method was simple,
reliable and sensitive.
出处
《上海医学检验杂志》
1999年第2期69-71,共3页
Shanghai Journal of Medical Laboratory Sciences
基金
上海市卫生系统百人计划培养经费资助
