全氟辛烷磺酸钾对胎鼠肝脏信号因子STAT3蛋白表达的影响

Study on the Effects of PFOS-K on the Expression of STAT3 Protein in Embryo of Mice

[目的]以胎鼠肝脏细胞中的信号转导及转录激活因子3(STAT3)蛋白表达的阳性细胞率(STAT3蛋白表达量)为指标,探讨全氟辛烷磺酸钾(PFOS-K)对小鼠胚胎的肝脏发育是否有影响,进而了解其胚胎毒作用。[方法]用染毒剂量分别为3、12mg/kg(按体重计)两个浓度的PFOS-K混悬液给怀孕9d的雌鼠灌胃,4d后(即怀孕第13.5天时)采用免疫组织化学法检测胎鼠肝脏的STAT3蛋白表达量。[结果]3、12mg/kg两个剂量组的阳性细胞表达率分别为41.62%和14.77%,差异有统计学意义(P<0.01)。[结论]PFOS-K能够影响小鼠胚肝脏发育过程中的信号转导因子STAT3蛋白的表达量,对哺乳动物的模式动物胎鼠肝脏细胞具有一定的胚胎毒作用。

[ Objective ] To study the effect of perfluorooctane sulfonate（ PFOS-K ）on the liver development in embryo of mice and the toxicity in the embryo. [ Methods ] PFOS-K solution with concentrations of 3mg/kg and 12 mg/kg in a cumulative dose series was infused through gavage into nine-day-pregnant mice. Then, 4 days after the treatment the expression of signal transduction and activators of transcription 3 （ STAT3 ） protein in the embryo liver were measured by immunohistochemistry. [ Results ] The expression rates of SATA3 protein in mice with dose 3 mg/kg and 12 mg/kg were 41.62% and 14.77%, respectively. The results showed significant difference（ P 〈 0.01 ）. [ Conclusion ] The results showed that PFOS-K could affect the expression amount of STAT3 protein in the embryo liver development. It could be concluded that PFOS-K has certain damage to the liver cell in embryo of mice.