经表面修饰的PLLA多孔材料修复骨缺损的实验研究

Experiment of surface modified poly L-lactic acid on repair of bone defects

目的:探讨经表面修饰的左旋聚乳酸(PLLA)多孔材料作为骨组织工程支架的可行性。方法:采用酶消化法和组织块法相结合的方法进行兔成骨细胞的体外分离和培养,将成骨细胞与PLLA材料共培养,通过倒置显微镜观察成骨细胞在材料表面的增殖能力,并将材料植入成年新西兰兔体内,术后30、60d观察大体形态、X线片及骨密度检测、骨生物力学检测结果。结果:(1)成功从新生兔颅骨组织块中分离培养出成骨细胞;(2)与材料共培养后,材料表面可见成骨细胞附着;(3)各组兔术后恢复及进食均正常,伤口无炎症反应,愈合良好;(4)大体观察、X线检查、放射学评分及生物力学检测显示,术后30、60d实验组与对照组评价骨生成差异有统计学意义(P<0.05)。植入GEL/HA涂层PLLA复合材料组术后60d骨缺损修复情况明显好于不植入任何材料组及单纯PLLA多孔材料组。结论:经表面修饰的PLLA多孔材料具备修复骨缺损的能力。

Objective：To evaluate the feasibility of poly L-lactic acid（PLLA）as tissue engineering bone material.Methods：We used the combined treatment of tissue block and enzyme digestion to isolate and culture the rabbit osteoblasts.The osteoblast was co-cultured with PLLA in vitro in rabbits,the cell-material complex was observed under a phase-contrast microscope.Then transplanted the PLLA with the rabbit in vivo.The specimens harvested at 30 and 60 days after operation were respectively evaluated by gross observation,X-ray examination,histology staining,and image pattern analysis.Results：（1）The osteoblasts were isolated and cultured successfully.（2）There were osteoblasts accreted on the surface of the stuff after they were cultured together.（3）The postoperative recovery and food intake were normal.The wound healed well and without inflammation present in the wound.The concrescence was fine.（4）Gross observation,X-ray examination,image pattern analysis and biomechanics examination showed that there were significant differences in bone regeneration between GEL/HA-PLLA group,PLLA group and negative group in the 30 and 60 days after operation（P〈0.05）.Conclusion：Surface modified poly L-lacticacid possesses the potency of repairing bone defects.