辐射与花药培养相结合筛选烟草抗黄瓜花叶病毒病突变体

CUCUMBER MOSAIC VIRUS RESISTANT MUTANT FROM TOBACCO'S ANTHERS TREATED WITH γ RAY THROUGH ANTHER CULTURE

用６０Ｃｏγ射线辐射处理小孢子处于单核靠边期的烟草Ｋ３２６、ＮＣ８９、８６１１花蕾并进行花药培养，获得的单倍体植株移栽成活后接种黄瓜花叶病毒病（ＣＭＶ）汁液。结果表明，ＮＣ８９、８６１１单倍体植株的抗病性变异与６０Ｃｏγ射线辐射剂量之间存在一定范围的定向性，剂量≥４ｋｒａｄ时，植株叶片变异与ＣＭＶ症状相似，不利于选择；剂量＜２ｋｒａｄ时，植株的抗病性与对照之间没有差异；剂量＝２ｋｒａｄ时，植株的抗病性与对照之间的差异可达到显著水平，并在２ｋｒａｄ处理的ＮＣ８９、８６１１单倍体植株群体中筛选到了抗ＣＭＶ突变体。

Flower buds of Nicotiana tabacum cv. K326,NC89 and 8611, in which the microspore is in the phase of single nucleus and the nucleus is on the side, were irradiated with 60 Co γ ray. Plantlets from anther culture were transplanted and inoculated with poisonous fluid of CMV. The results showed that there was an orientation relation between the variation of disease resistance of plants of NC89, 8611 and the dose of 60 Co γ ray. The variation of leaves of plants was analogous to symptom of CMV when the dose was equal to or more than 4, which caused difficulty for selection. When dose was less than 2krad, there was no difference in disease resistance between the plants induced from irradiation of 60 Co γ ray and the control. However, when dose was equal to 2krad, the difference of disease resistance was at the level of significance. CMV resistant mutants were selected from the population of haploid plant of NC89 and 8611 induced by 2 0krad of 60 Co γ ray.