全血体系下草鱼红细胞免疫粘附功能及其调控因素研究

Study on the Immune Adherence Function and Regulation of Grass Carp(Ctenopharyngodon idellus)Erythrocytes

采用经典的红细胞C3b受体(erythrocyte C3b receptors,E-C3bR)与红细胞免疫复合物(erythrocyte immune complexes,E-IC)花环实验,检测草鱼红细胞在全血体系与洗涤红细胞体系下的E-C3bR与E-IC粘附率,同时检测未灭活血清与灭活血清(58℃处理30min)对洗涤草鱼红细胞免疫粘附功能的调控作用.结果显示:全血体系下草鱼红细胞E-C3bR粘附率与E-IC粘附率均显著高于洗涤红细胞体系条件下的相应值(p<0.05).进一步研究显示,灭活血清组处理的洗涤草鱼红细胞E-C3bR粘附率与E-IC粘附率显著高于未灭活血清组及生理盐水组(p<0.05);未灭活血清组处理的洗涤草鱼红细胞E-C3bR粘附率与E-IC粘附率显著低于生理盐水组(p<0.05).结果提示,草鱼红细胞的免疫粘附功能受全血反应体系中相关因子的调控,其血清中存在热不稳定的抑制因子和热稳定的促进因子.

Using classical erythrocyte C3b receptor （E-C3bR） rosettes and erythrocyte immune complexes （E-IC） rosettes,the immune adherence rates of grass carp （Ctenopharyngodon idellus） erythrocytes to non-sensitized （E-IC） and sensitized yeasts （E-C3bR） were investigated in whole blood conditions and in washed conditions. The washed grass carp erythrocytes were treated with autologous serum or autologous inactivated serum（58 ℃ 30 min）and 0.68 % physiological saline. The results indicated that the rates of E-C3bR rosettes and E-IC rosettes in whole blood cells （WBC） were both significantly higher than those in washed red blood cells （WRBC） （p0.05）. Further study showed that the rates of E-C3bR rosettes and E-IC rosettes of the washed erythrocytes which were treated with autologous inactivated serum （58 ℃ 30 min） were significantly higher than those of the washed erythrocytes treated with autologous serum and 0.68% physiological saline （the control group） （p0.05）. Meanwhile,the rates of E-C3bR rosettes and E-IC rosettes of the washed erythrocytes which were treated with autologous serum were significantly lower than those of the control group （p0.05）. These results suggested that the immune adherence function of grass carp erythrocytes might be regulated by the relevant factors of the whole blood,and both thermo-stable enhancer and thermo-labile inhibitor exist in grass carp sera.