小麦族内几种远缘禾草及其杂交种过氧化物酶同工酶分析

The Analysis on Peroxidase (POD) Isozyme of Several Wide Species and Their Hybrids in the Triticeae.

采用聚丙烯酰胺垂直板电泳技术对小麦族内的加拿大披碱草、野大麦及其杂种Ｆ１和蒙古冰草、航道冰草及其杂种Ｆ１、ＢＣ１的过氧化物酶（ＰＯＤ）同工酶做了比较研究。结果表明，每种植物的ＰＯＤ酶谱可明显分成Ａ、Ｂ两区，在Ｂ区有２条位点相同的酶带，为亲本的基带；所有供试材料呈现６～９条酶带，亲本与其杂种后代的酶谱表型有一定差异；ＰＯＤ同工酶具多态性，可作遗传标记用于杂种鉴定和回交后代目标性状植株的检测；加拿大披碱草×野大麦Ｆ１具双亲各自酶谱的强带，可能Ｆ１具两亲本的某些优良性状。

The POD isozyme of Elymus canadensis, Hordeum brevisbulatum and their F 1 hybrid, Agropyron mongolicum, Agropyron cristatum and their F 1 hybrid and backcross 1 in Triticeae were analyzed by polyacrylamide gel electrophoresis. The results showed: the POD zymograms of each species can be divided into two regions (A and B). There were two isozyme bands at same location, which were parental base bands. All experiment materials had 6～9 isozyme bands. There were differences in the zymograms between parents and their hybrids. There was polymorphism in the POD isozyme and it can be used as genetic marker to identify hybrid and to determine the plants with object characteristics in backcross descendants. The E. canadensis-H. brevisubulatum F 1 hybrid had parental isozyme bands with higher activity. It probably possesses of some superior characteristics of parents.