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AF4通道技术表征病毒样颗粒

Virus-Like Particle Characterization Using AF4 Channel Technology
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摘要 病毒样颗粒(VLP)是一种越来越受到广泛关注的抗体疫苗。为了对其进行质量控制,采用灵敏的分析方法非常重要。这项研究阐述了将非对称流分离(AF4)方法开发用于VLP研究。结果表明,AF4在研究VLP表达方面具有很大的潜力,超过了通常使用的动态光散射和尺寸排阻高效液相色谱法。 Virus-like particles （VLPs） are a class of vaccines of growing interest. For quality control purposes, the application of sensitive analytical methods is very important. This study illustrates the development of asymmetric flow field-flow fractionation （AF4） methods for the investigation of VLP. The results demonstrate the potential of AF4 to investigate VLP formulations, and the method is shown to exceed the capabilities of commonly applied methods such as dynamic light scattering （DLS） and size exclusion （SE）-HPLC.
作者 Rainer LANG Lorenz VOGT Alexander ZüRCHER Gerhard WINTER
出处 《生命科学仪器》 2010年第4期10-12,共3页 Life Science Instruments
关键词 病毒样颗粒 通道技术 高效液相色谱法 表征 动态光散射 质量控制 流分离 非对称
分类号 O657.72 [理学—分析化学]
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生命科学仪器
2010年 第4期

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