摘要
本文利用自主研制的法医DNA提取纯化试剂盒在Hamilton Microlab~ Starlet DNA自动提取仪上提取276份血斑样本DNA,PCR扩增检测到270份样本STR分型,成功率达97.8%。结果表明法医DNA提取纯化试剂盒可与国外进口的DNA自动提取仪相配伍进行血斑样本的批量提取。
In the study,forensic DNA extraction and purification kit was used to extract a total of 276 blood sample DNA from DNA database on Hamilton Microlab~ Starlet DNA automation workstation.All sample DNA was amplified by PCR.STR genotyping for 270 samples was detected,and the success rate was up to 97.8%.The results indicated that forensic DNA extraction and purification kit can be applied to accomplish lots of sample DNA extraction in coordination with foreign DNA automation workstation.
出处
《生命科学仪器》
2010年第4期54-56,共3页
Life Science Instruments
基金
国家"十一五"科技支撑计划项目课题"法医学DNA提取
纯化试剂及DNA提取仪的研制"(课题编号:2007BAK26B03)
