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西洋参茎叶皂苷对大鼠局灶性脑缺血后半胱氨酸天冬氨酸蛋白酶8表达的影响 被引量：1

Effects and further mechanism of Panax quinquefolium saponins from steams and leaves on caspase-8 after cerebral ischemia in rats
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摘要 目的研究西洋参茎叶皂苷(PQS)对大鼠局灶性脑缺血后半胱氨酸天冬氨酸蛋白酶8(caspase-8)表达的影响,并进一步探讨其机制。方法采用线栓法阻断大鼠一侧大脑中动脉(MCAO),制备局灶性脑缺血模型,逆转录聚合酶链反应(RT-PCR)技术检测大脑皮质凋亡相关基因caspase-8mRNA表达。结果模型组caspase-8mRNA表达较PQS高剂量组、PQS低剂量组、尼莫地平组、假手术组均明显增加(0.713±0.117 vs 0.467±0.089、0.552±0.099、0.468±0.097、0.422±0.077,P<0.01);而其他组间caspase-8mRNA表达差异均无统计学意义。结论PQS对大鼠局灶性脑缺血具有保护作用,其机制可能与西洋参茎叶皂苷抑制神经元细胞凋亡有关。 Objective To investigate the effects and the further mechanism of Panax quinquefolium saponins（PQS） from steams and leaves on caspase-8 after cerebral ischemia in rats.Methods The rats with incomplete cerebral ischemia were induced with fishing lines in the middle cerebral artery（MCA）.The caspase-8 mRNA in the cortical neuron was detected with reverse transcription polymerase chain reaction（RT-PCR）.Results The caspase-8mRNA expression of the study group was significantly higher than that of high-dose PQS,low-dose PQS,nimodipine and sham-operation groups（0.713±0.117 vs 0.467±0.089,0.552±0.099,0.468±0.097,0.422±0.077,P0.01）.Conclusion PQS had protecting effect on cerebral ischemia.The mechanism of PQS against cerebral ischemia is related to inhibiting cell apoptosis.
作者 李增宁 关利新 焦广宇 魏新刚 苏云明
机构地区 河北医科大学第一医院营养科 牡丹江医学院药理教研室 黑龙江省医院营养科 黑龙江省卫生厅 黑龙江中医药大学药理教研室
出处 《临床荟萃》 CAS 2010年第18期1577-1579,共3页 Clinical Focus
基金 黑龙江省自然科学基金课题(ZA2006-07)
关键词 脑缺血 西洋参 细胞凋亡 半胱氨酸天冬氨酸蛋白酶8 brain ischemia panax quinquefolium apoptosis caspase-8
分类号 R742 [医药卫生—神经病学与精神病学]
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参考文献10

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二级参考文献34

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临床荟萃
2010年 第18期

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