血卟啉单甲醚介导的光动力作用对小鼠肝癌细胞生长抑制的初步研究

Photodynamic inhibition of hematoporphyrin monomethyl ether in hepatoma cells

目的：观察血卟啉单甲醚介导的光动力作用对小鼠肝癌H22细胞生长的抑制作用。方法：采用倒置显微镜观察和MTT法检测血卟啉单甲醚介导的光动力作用后小鼠肝癌H22细胞的生长情况。结果：血卟啉单甲醚介导的光动力作用显著抑制小鼠肝癌H22细胞的生长,在一定光能密度条件下,细胞生长抑制作用跟血卟啉单甲醚浓度间呈剂量依赖关系。倒置显微镜下观察到光动力作用组癌细胞数量显著减少,细胞固缩,而单纯光照组,单纯血卟啉单甲醚组和假照射组三组间无明显差异;MTT法示光动力组细胞抑制率显著高于单纯光照组、单纯血卟啉单甲醚组和假照射组（P〈0.05）,单纯血卟啉单甲醚组和假照射组间无明显差异（P〉0.05）。结论：血卟啉单甲醚光动力学作用对小鼠肝癌细胞H22生长抑制作用确切。

Objective：To explore photodynamic inhibition of Hematoporphyrin Monomethyl Ether（HMME） in Hepatoma Cells.Methods：The photocytotoxic potency and efficacy of HMME 24h after PDT in hepatoma cell line H22 was investigated by inverted microscope and MTT reduction assay.Results：Inverted microscope and MTT reduction assay showed that the photodynamic inhibition of HMME in H22 was extraordinarily significant.The photocytotoxicity of HMME indicated dose dependent characteristics in H22 cells.Conclusion：HMME induced PDT could effectively kill H22 cells and HMME will become an alternative photosensitizer for photodynamic therapy.