稀土荧光螯合物时间分辨荧光光谱分析方法研究

Study on Method of Time-Resolved Fluorescence Spectra for RareFluorescence Chelate

以提高固相时间分辨荧光免疫分析灵敏度为目的,在自行研制固相时间分辨荧光免疫分析样机和合成新荧光化合物的基础上,首先对新合成的4,4′-二溴-6,6′-二(N,N-二(乙氧基羰基)氨甲基)-2,2′-联吡啶-Eu(Ⅲ)螯合物的荧光性质及时间分辨荧光光谱分析方法进行了研究;其次对Eu-TTA-phen和Eu-β-NTA-TOPO等化合物进行时间分辨荧光光谱分析检测。

In the present paper,the technology of time-resolved fluorescence spectra was investigated in order to improve the sensitivity of the solid-phase time-resolved fluorescence immunoassay.The fluorescence properties,fluorescence intensity and detection method of the novel complexes 4,4′-dibromo-6,6′-bis（N,N-bis（ethoxycarbonylmethyl）amino methyl）-2,2′-bipyridine were investigated through self-developed solid-phase time-resolved fluorescence immunoassay instrument.At the same time,the Eu-TTA-phen and Eu-β-NTA-TOPO series of Eu（Ⅲ） compounds were detected.