Toll样受体2和4在早产儿败血症中的临床意义

The Clinical Significance of Toll-like Receptor 2 and 4 in the Sepsis of Preterm Children

目的:探讨Toll样受体2、4在早产儿炎性免疫反应发病机制中的作用。方法:选取败血症早产儿21例为败血症组,同期住院非炎症疾病早产儿17例为对照组。抽取各组患儿外周静脉血0.5mL,抗凝,密度梯度离心法分离其外周血单个核细胞(PBMC),采用实时荧光定量PCR检测PBMC内TLRs信号传导途径分子TLR2、TLR4mRNA的表达。结果:败血症组患儿PBMCTLRs信号传导途径分子TLR2、TLR4mRNA表达与对照组比较显著增高(P<0.05)。G+菌感染败血症组,TLR2mRNA表达较TLR4mRNA水平升高(P<0.05),而在G-菌感染菌血症组,TLR4mRNA表达水平较TLR2mRNA升高(P<0.05)。结论:败血症患儿存在TLR2和4信号传导途径分子的异常活化。TLR2和TLR4可以作为早产儿败血症早期检测指标之一。

Objective To study the role of Toll-like receptor 2 and 4 in the pathogenesis of inflammatory immune response in preterm children.Methods 21 cases of sepsis in premature children were the sepsis group,and 17 cases of hospital premature children without inflammatory disease over the same period were as the control group.Children in each group were collected peripheral venous blood 0.5 mL with anticoagulation,and using density gradient centrifugation for separation of peripheral blood mononuclear cells （PBMC）.Then using real time quantitative PCR to detect TLR 2 and TLR 4 mRNA expression in TLRs signaling pathway of PBMC.Results Compared with the control group,TLR 2 and TLR 4 mRNA expression of PBMC was significantly higher in sepsis group patients （P 0.05）.Conclusion There are abnormal activation of signaling pathway molecular TLR 2 and TLR 4.TLR 2 and TLR 4 may as one of early examination targets of sepsis in premature children.