小檗碱对体外培养大鼠大脑皮层神经元损伤的保护作用

Protective Effects of Berberine on Neuronal Injury in Cultured Cortical Neurons in Rats

体外培养大鼠胎鼠大脑皮层神经细胞，观察小檗碱（ｂｅｒｂｅｒｉｎｅ，Ｂｅｒ）对Ｎ－甲基－Ｄ－门冬氨酸（Ｎ－ｍｅｔｈｙｌ－Ｄ－ａｓｐａｒ－ｔａｔｅ，ＮＭＤＡ）、过氧化氢（Ｈ２Ｏ２）及撤血清培养引起的细胞损伤的保护作用。结果表明，培养的大鼠胚胎大脑皮层神经细胞在ＮＭＤＡ５００μｍｏｌ／Ｌ作用１０ｍｉｎ、或Ｈ２Ｏ２１００μｍｏｌ／Ｌ２４ｈ、或无血清培养４８ｈ后，细胞的死亡率及乳酸脱氢酶（ＬＤＨ）漏出率明显增加，抗氧化酶ＳＯＤ、谷胱甘肽过氧化酶（ＧＳＨ－Ｐｘ）活性显著降低，而脂质过氧化物丙二醛（ＭＤＡ）生成增多。Ｂｅｒ１０、３０μｍｏｌ／Ｌ能显著降低细胞死亡率、ＬＤＨ漏出率及ＭＤＡ的生成，且能明显提高抗氧化酶活性。提示：Ｂｅｒ可拮抗ＮＭＤＡ、Ｈ２Ｏ２及撤血清培养引起的神经细胞损伤，其机理可能与其减少膜脂质过氧化物生成。

The protective effects of berberine (Ber) on neuronal injury induced by NMDA H 2O 2 or serum free culture were observed in cultured cortical neurons of rat embryo. When cultured neurons were incubated for 10 min with NMDA 500 μmol/L、 or 24 h with H 2O 2 100 μmol/L or 48 h with serum free cultures, efflux of LDH and content of MDA increased while the number of surviving neurons and the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH Px) decreased. Ber 10, 30 μmol/L reduced the percentage of cell death and LDH content in extra cellular bathing media in injuried cortical cultures. It also prevented the elevation of MDA and increased the activity of SOD and GSH Px. These results suggested that Ber could prevent the toxicity of NMDA and H 2O 2 or serum free culture insults by suppressing the generation of lipid peroxide and increasing the activities of antioxidant enzymes. [WT5,5”HZ]