胰岛素对牛主动脉平滑肌细胞前列环素生成的影响

Effects of Insulin on Prostacyclin Production from Bovine Aortic Smooth Muscle Cells

应用细胞培养技术对新生牛主动脉平滑肌细胞进行传代培养，取第４～６代平滑肌细胞进行实验，研究不同时间、不同浓度胰岛素对平滑肌细胞前列环素（ｐｒｏｓｔａｃｙｃｌｉｎ，ＰＧＩ２）生成的影响。分别于加入胰岛素培养０、６、１２、２４ｈ收集细胞上清液，并将各孔细胞计数，采用放免法测定细胞上清液中６－ｋｅｔｏ－ＰＧＦ１α含量（６－ｋｅｔｏ－ＰＧＦ１α是前列环素的稳定代谢产物）。结果显示：加入胰岛素培养１２ｈ与２４ｈ，胰岛素浓度与细胞上清液中６－ｋｅｔｏ－ＰＧＦ１α含量呈负相关（１２ｈ：ｒ＝－０．７９８，Ｐ＜０．００１；２４ｈ：ｒ＝－０．８６６，Ｐ＜０．００１）。

The bovine aortic smooth muscle cells were cultured from generation to generation. We investigated the effects of different concentrations of insulin on prostacyclin (PGI 2) production of smooth muscle cells (passage 4￣6) on different periods of incubation. Each well was incubated with 1 ml of culture medium containing insulin. At the end of 0 h, 6 h, 12 h, 24 h incubation, the supernatants were collected from the culture and the cells were counted in each well. The concentrations of 6 keto PGF 1α (the stable PGI 2 metabolite) in the culture medium (supernatant) were determined by a specific radioimmunoassay. The results showed that at the end of 12 h and 24 h incubation with insulin, there were significant negative correlations between insulin concentrations and 6 keto PGF 1α contents of supernatants of smooth muscle cells (12 h : r =-0.789, P <0.001; 24 h : r =-0.866, P <0.001). The results suggest that insulin may inhibit secret of prostacyclin by smooth muscle cells.