蕈样肉芽肿外周血淋巴细胞CD28分子表达的研究

Study of CD28 Molecule Expression on Lymphocytes of Peripheral Blood in Patients with Mycosis Fungoides

目的：探讨ＣＤ２８分子在蕈样肉芽肿（ＭＦ）肿瘤免疫中的作用。方法：采用免疫荧光双标记—流式细胞仪检测１０例ＭＦ病人外周血淋巴细胞的ＣＤ２８分子。结果：ＭＦ病人外周血ＣＤ２８＋淋巴细胞为（２５．０６±５．８９）％，低于正常对照组（７０．６２±７．８３）％有非常显著性差异（Ｐ＜０．００１）；ＣＤ２８＋淋巴细胞数与ＭＦ临床分期呈显著直线负相关（ｒ＝－０．７８５，Ｐ＜０．０５）；ＭＦ病人外周血ＣＤ—ＣＤ２８＋淋巴细胞为（５．８５±０．５２）％，低于对照组（１３．２６±４．６９）％，有非常显著性差异（Ｐ＜０．００１）；ＣＤ８＋—ＣＤ２８＋淋巴细胞与ＭＦ的临床分期呈显著直线负相关（ｒ＝－０．９３９，Ｐ＜０．００１）。结论：ＭＦ病人外周血淋巴细胞ＣＤ２８分子表达存在异常。

Objective:To explore the effect of CD28 molecule on tumor immunity of mycosis fungoides (MF).Methods:By immunofluorescent double marker flow cytometry,we detected the CD28 molecule on lymphocytes of peripheral blood for 10 cases of MF.Results:The number of CD28 + lymphocytes in peripheral blood for MF patients was (25.06±5.89)%,which was significantly lower than that of the control group (70.62±7.83)%,P<0.001,There was negative correlation between the number of CD28 + lymphocytes and MF clinical stages, r=-0.785,P<0.05. The number of CD8 + CD28 + lymphocytes was (5.85±0.52)%, which was remarkably lower than that of the control group (13.26±4.69)%,P<0.001.There was significantly negative correlation between the number of CD8 + CD28 + lymphocytes and MF clinical stages,r=-0.939,P<0.001. Conclusion:There existed the abnormal expression of CD28 molecule on the lymphocytes in peripheral blood for MF patients,which might have relationship with the pathogenesis of MF.