1995～1997年辽宁、河南、河北省麻疹病毒流行株基因型分析

Genotic Analysis of Wild Type Measles Viruses Circulating in Iiaoning,Henan,Heibei Provinces of China During 1995～1997.

以反转录二步多聚酶链反应方法（ＲＴ－ＰＣＲ）直接测定麻疹病人唾液、尿液标本中的麻疹病毒（ＭＶ）血凝基因（Ｈ）、核衣壳基因（Ｎ）。以ＲＴ－ＰＣＲ产物纯化后进行ＭＶ４５０ｎｔＨ、２８２ｎｔＮ基因核苷酸片段测序分析，测序结果一致表明１９９５～１９９７年期间中国部分地区ＭＶ流行株存在２种基因型。其中一种基因型只发现１株，与ＥＤｗ５４株相同，另一种基因型占流行株的绝大多数（称新基因型），与其它国家以前报道的ＭＶ流行株基因型的Ｎ基因最大差异为１５．４％，与ＥＤｗ５４、疫苗株相差８．８％；Ｈ基因最大差异是１０．８％

To investigate the molecular epidemiology of measles virus (MV) in china,sequence analysis of MV was performed based on a 282 nt region of the nucleoprotein (N) gene and a 450 nt region of the heamaglutinin (H) gene by direct sequencing of RT PCR amplicons obtained from clinical specimens.The results revealed that one MV strain belongs to ED like genotype and remainder strains were clustered into a new genotype,which was distinct from Chinese vaccine strains and MV strains previously described circulating in other countries,and represented a new genotype.There was up to 15 4% divergence in the nt 282 N gene and 10 8% divergence in the nt 450 h gene between the new genotype and other genotypes.