氯化镍对人红细胞膜损伤机理的波谱学研究

Spectrum studies on the mechanisms of human erythrocyte membrane injuries induced by nickel chloride

为探讨镍对红细胞膜损伤作用的机理，采用傅立叶变换红外吸收技术和园二色光谱技术，分别研究了氯化镍对人红细胞膜蛋白和重组于卵磷脂脂质体上ｂａｎｄ３蛋白的二级结构影响，同时采用５－脱氧不饱和硬脂酸标记的电子自旋共振技术，研究了膜脂质流动性的变化．结果表明：在１－１００μｍｏｌ·Ｌ－１浓度范围内，镍离子可使红细胞膜蛋白发生变性和不可逆聚合，使膜浅层脂质流动性增加；０．０５，０．１０，０．５０ｍｍｏｌ·Ｌ－１的镍可使ｂａｎｄ３的α－螺旋和无规卷曲含量分别有减少和增加的趋势．

Changes of conformation of human erythrocyte membrane proteins were investigated with Fourier transform infrared absorbance spectroscopy and circular dichroism. The fluidity of membrane lipid was studied with 5 deoxyl stearic acid labeling electron spin resonance spectroscopy. The results showed that nickel chloride aqueous solution caused the denaturation and coagulation of membrane proteins as well as the increase of membrane lipid fludity at concentration of 1.0-100 μmol·L 1 ; at concentration of 0.05, 0.1, 0.5 mmol·L 1 , nickel might induce the change of the secondary structure, there were tendencies to reduction of α helix content and increase of the content of unordered structure.