宫颈癌p53蛋白表达和细胞增殖活性

Expression of p53 protein and cellular proliferative activity in cervix carcinoma

目的：研究p53蛋白，PCNA，DNA含量在宫颈癌及癌前病变组织中的表达及其意义．方法：用免疫组化ABC法和图像分析法，检测p53蛋白、PCNA和DNA含量，对56例正常宫颈粘膜、宫颈上皮内瘤变、宫颈浸润性鳞癌进行研究．结果：p53表达阳性率、PCNA细胞增殖指数和DNA含量随癌组织分级依次增高，p53蛋白在正常粘膜不表达，在宫颈上皮瘤变和原位癌少量表达，在宫颈浸润癌表达明显增高．结论：p53含量和细胞活性增高与宫颈粘膜癌变发生及恶性程度有关．PCNA增殖指数、DNA含量和多倍体细胞的出现，对宫颈癌的早期诊断有一定意义．p53蛋白的阳性表达在宫颈癌发生中不是一个晚期事件．

AIM: To investigate the expression of p53 proteinand cellular proliferative activity in cervix carcinoma, we determined the p53 protein, PCNA and nuclear DNA content incervix intraepithelial neoplasia (CIN), in situ carcinoma andcervix squamous cell carcinoma. METHODS: Materials obtained from 5 CIN, 10 in situ carcinomas and 56 squamouscarcinomas were determined by using immunohistochemistryfor expression of p53 protein and PCNA, and image cytometry for DNA content and ploidy. RESULTS: The resultsshowed that increasing histopathological atypia in cervix mucosa was correlated to an DNA content followed by p53 overexpression. CONCLUSION: The data are suggested to contribute to a better understanding of the genesis of cervix carcinoma, and indicate that the increase of DNA content andhyperploid as well as increase of PCNA proliferating index canbe used to decide if a cell population is neoplastic. The resultsalso indicated that alterations in p53 protein expression is notan early event in pathogenesis of cervix neoplasia, since expression of p53 protein was not present in normal mucosa,and was only rarely observed in CIN and in situ carcinoma,while it was common in invasive carcinomas of the cervix immunohistochemistry.