κ阿片受体介导100 Hz电针对吗啡戒断大鼠心动过速的缓解作用

100 Hz electroacupuncture amelioration of tachycardia of morphine withdrawal rats mediated by κ opioid receptor in central nervous system

目的：研究１００Ｈｚ电针缓解吗啡戒断大鼠心动过速的受体机制。方法：在吗啡戒断１８～２４ｈ的大鼠模型上，腹腔注射阿片受体拮抗剂纳络酮（ｎａｌｏｘｏｎｅ，ＮＸ）或侧脑室注射κ阿片受体拮抗剂ｎｏｒｂｉｎａｌｔｏｒｐｈｉｍｉｎｅ（ｎｏｒＢＮＩ），给予１００Ｈｚ电针刺激，记录吗啡戒断大鼠清醒状态下的心率和血压。结果：１００Ｈｚ电针的作用可被腹腔注射ＮＸ１ｍｇ·ｋｇ－１完全翻转、被侧脑室注射ＮｏｒＢＮＩ１２ｎｍｏｌ完全阻断。结论：１００Ｈｚ电针抑制吗啡戒断大鼠心动过速的作用主要是通过中枢κ阿片受体介导的。

Objective: To study the receptor mechanism of 100 Hz electroacupuncture (EA) amelioration of tachycardia of morphine withdrawal rats. Methods: Morphine dependence was induced in Wistar rats using 8 day morphine injection regime. Heart rate and blood pressure of the rats were recorded after 18 24 h of morphine abstinence, and 100 Hz EA stimulation was then given. Results: Tachycardia of the rats was ameliorated by 100 Hz EA, those effect was reversed by injecting opioid receptor antagonist naloxone (i.p. at 1 mg·kg -1 ), or by administrating selective κ opioid receptor antagonist nor BNI (intracerebro ventricular injection i.c.v. at 12 nmol). Conclusion:The effect of 100 EA in ameliorating tachycardia during morphine abstinence was mediated by κ opioid receptors in CNS.