碱性成纤维细胞生长因子对大鼠血管球囊成形术后基质金属蛋白酶表达的影响

Expression of matrix metalloproteinases mRNA induced by basic fibroblast growth factor in the rat artery after balloon angioplasty

目的：探讨碱性成纤维细胞生长因子对基质金属蛋白酶表达的影响及其在球囊术后再狭窄中的作用。方法：２４只雄性Ｗｉｓｔａｒ大鼠分成对照组、球囊损伤组（１４ｄ）及实验组。正常组不做处理；球囊损伤组行单纯球囊拉伤，术后１４ｄ取材；实验组大鼠胸主动脉球囊成形，术后即刻、３、６、９ｄ分别尾静脉注射１０、５、３、２μｇ碱性成纤维细胞生长因子，１４ｄ处死取材，Ｎｏｒｔｈｅｒｎ杂交观察基质金属蛋白酶１、２及９的ｍＲＮＡ的改变。结果：正常对照组，基质金属蛋白酶１、２及９均无明显表达。球囊损伤组，只有基质金属蛋白酶９有较少量表达；实验组基质金属蛋白酶１明显表达，基质金属蛋白酶９表达较球囊损伤组升高，基质金属蛋白酶２仍无明显表达。结论：碱性成纤维细胞生长因子能选择性促进成形术后基质金属蛋白酶的表达，可能在调节细胞外基质的降解。

Objective: To investigate the effect of basic fibroblast growth factor (bFGF) on matrix metalloproteinases (MMPs) and restenosis after PTCA. Methods: bFGF was injected through the tail vein at once (10 μg), day 3(5 μg), day 6(3 μg), day 9(2 μg) after balloon injury in the thoracic aorta of rats. The rats were killed and vessels were harvested after two weeks. Northern blot was used to investigate the expression of MMP1, MMP2 and MMP9 mRNA in the thoracic aorta. Results: No MMPs mRNA expression was observed in the normal artery. Expression of MMP9 mRNA was detected two weeks after balloon angioplasty without expression of MMP1, MMP2 mRNA. Significant expression of MMP1 was detected and MMP9 increased much in the rats given bFGF. Conclusion: bFGF can induce expression of MMPs mRNA selectively after balloon angioplasty. It may play a very important role in the degradation of extracellular matrix and remolding after angioplasty.