致鸡产蛋下降禽病原的检测基因诊断芯片的研制及临床应用

Establishment and diagnostic assay for egg drop syndrome diseases with gene chip

本试验研究的目的是利用RT-PCR技术扩增出新城疫病毒(NDV)、低致病性禽流感病毒(AIV H9)、传染性支气管炎病毒(IBV)、减蛋综合征(EDS)、传染性喉气管炎病毒(ILT)、鸡毒支原体(MG)各一段保守序列,以纤维素酯膜作为生物芯片制作的基质,通过微量点样技术制作基因芯片(gene chip)。从可疑病料中提取组织核酸,通过掺入法bio-11-dUTP标记,对杂交信号的强弱检测来实现对生物样品的高效分析诊断。

With polymerase chain reaction or reverse transcription-polymerase chain reaction the fragments of newcastle disease virus（NDV）,avian influenza virus（AIV Hg）,infectious bronchitis virus（IBV）, egg drop syndrome（EDS）, infectious laryngotracheitis virus（ILTV）,Mycoplasma gallisepticum（MG） were amplified with bio-11-dUTP labelibg and spotted onto colloxylin to form microarrays. The gene chips were subjected to specific hybridization and the hybridization results were scanned and analyzed with a scanner. The results showed that the gene chip could rapidly identify and rapidly distinguish the above-mentioned infectious diseases.