摘要
目的:研究α苦瓜子蛋白(αMMC)在HIV1急性和慢性感染T淋巴细胞中的抗HIV1活性.方法:以合胞体抑制实验,HIV1p24核心抗原表达水平和HIV抗原阳性细胞的百分率检测了αMMC的抗HIV活性.结果:αMMC对HIV1ⅢB诱导C8166细胞形成合胞体有显著的抑制作用.αMMC显著地抑制了HIV1急性感染T细胞中p24抗原的表达水平和减少了HIV抗原阳性细胞的百分率.在上述三种测定中,αMMC的EC50分别是0016,007和032mg·L-1.但是,αMMC不影响慢性感染HIV1ⅢB/H9中p24抗原表达水平.结论:αMMC是苦瓜子蛋白中唯一的具有抗HIV1活性的成份.αMMC抑制急性感染中HIV1的复制而对慢性感染T细胞中的HIV1复制无影响.
AIM: To identify the anti human immuno ̄deficiency virus type 1 (HIV 1) activities of α momorcharin (α MMC) from Momordica charantia in acutely and chronically infected T lymphocytes. METHODS: The anti HIV ac ̄tivi ̄ties of α MMC were examined by 1) the inhibition of syncytia formation induced by HIV 1 ⅢB; 2) reduction of p24 core antigen expression level and decrease in numbers of HIV antigen positive cells in acutely and chronically infected cultures. The cytotoxic effects of α MMC was tested by trypan blue dye exclusion or colorimetric MTT assay. RESULTS: α MMC was found to obviously inhibit HIV 1 ⅢB inducing C8166 syncytia formation and markedly reduced both expression of p24 core antigen and the numbers of HIV antigen positive cells in acutely but not chronically HIV 1 infected culture. The median effective concentration (EC 50 ) in these assays were 0 016, 0 07, and 0 32 mg ·L -1 , respectively. CONCLUSION: α MMC is a unique component of momorcharin with anti HIV activity, and markedly inhibited HIV 1 replication in acutely but not chronically HIV 1 infected T lympho ̄cytes.
出处
《中国药理学报》
CSCD
1999年第3期239-243,共5页
Acta Pharmacologica Sinica
关键词
α-苦瓜子蛋白
T细胞
艾滋病毒
抑制作用
α momorcharin
ribosome inactivating proteins
HIV
antigen p24
giant cells
T lymphocytes
anti HIV agents
cytotoxi ̄city