Alpha-momorcharin inhibits HIV-1 replication in acutely but not chronicaly infected T-lymphocytes

Alpha momorcharin inhibits HIV 1 replication in acutely but not chronically infected T lymphocytes 1

目的：研究α苦瓜子蛋白（αＭＭＣ）在ＨＩＶ１急性和慢性感染Ｔ淋巴细胞中的抗ＨＩＶ１活性．方法：以合胞体抑制实验，ＨＩＶ１ｐ２４核心抗原表达水平和ＨＩＶ抗原阳性细胞的百分率检测了αＭＭＣ的抗ＨＩＶ活性．结果：αＭＭＣ对ＨＩＶ１ⅢＢ诱导Ｃ８１６６细胞形成合胞体有显著的抑制作用．αＭＭＣ显著地抑制了ＨＩＶ１急性感染Ｔ细胞中ｐ２４抗原的表达水平和减少了ＨＩＶ抗原阳性细胞的百分率．在上述三种测定中，αＭＭＣ的ＥＣ５０分别是００１６，００７和０３２ｍｇ·Ｌ－１．但是，αＭＭＣ不影响慢性感染ＨＩＶ１ⅢＢ／Ｈ９中ｐ２４抗原表达水平．结论：αＭＭＣ是苦瓜子蛋白中唯一的具有抗ＨＩＶ１活性的成份．αＭＭＣ抑制急性感染中ＨＩＶ１的复制而对慢性感染Ｔ细胞中的ＨＩＶ１复制无影响．

AIM: To identify the anti human immuno￣deficiency virus type 1 (HIV 1) activities of α momorcharin (α MMC) from Momordica charantia in acutely and chronically infected T lymphocytes. METHODS: The anti HIV ac￣tivi￣ties of α MMC were examined by 1) the inhibition of syncytia formation induced by HIV 1 ⅢB; 2) reduction of p24 core antigen expression level and decrease in numbers of HIV antigen positive cells in acutely and chronically infected cultures. The cytotoxic effects of α MMC was tested by trypan blue dye exclusion or colorimetric MTT assay. RESULTS: α MMC was found to obviously inhibit HIV 1 ⅢB inducing C8166 syncytia formation and markedly reduced both expression of p24 core antigen and the numbers of HIV antigen positive cells in acutely but not chronically HIV 1 infected culture. The median effective concentration (EC 50 ) in these assays were 0 016, 0 07, and 0 32 mg ·L -1 , respectively. CONCLUSION: α MMC is a unique component of momorcharin with anti HIV activity, and markedly inhibited HIV 1 replication in acutely but not chronically HIV 1 infected T lympho￣cytes.