以神经特异性标记识别成年大白鼠大脑皮层损伤引起的细胞分裂

IDENTIFICATION OF INJURY-INDUCED MITOTIC CELLS IN ADULT RAT CEREBRAL CORTEX BY NEURON-SPECIFIC MARKERS

切除成年大白鼠双侧大脑皮层顶叶正中部分,术后第四天取损防区及附近的大脑组织,用烯醇化酶(NSE)抗体和神经丝(NF)抗体免疫组织化学法显示了分裂神经元的形态。30只大白鼠用NSE免疫染色,有15只发现部分免疫反应阳性细胞有分裂象。25只大白鼠经NF免疫染色,有10只发现部分免疫反应阳性细胞有分裂象。进行分裂的神经元是颗粒细胞及小型和中型的锥体细胞,分布在损伤区边缘100—500 μm范围之内,以皮层的第Ⅲ层出现较多。本文结果提示在大脑皮层损伤条件下损伤区旁神经元出现分裂象,可能是神经元可塑性反应。

Adult rat brains were injured by partial decortication in the parietal regions. Four days later. the brains were examined for mitotic cells that were immunostained for neuronal markers, the neuron-specific enolase (NSE) and neurofilaments (NF). Of the 30 rats stained for NSE, 15 were found to have NSE-positive cells containing mitotic figures; of the 25 rats stained for NF, 12 possessed NF-positive cells in mitosis. Most of the neurons in mitosis were granule cells and small-and medium size-pyramldal cells and were located in cortical layer Ⅲ within 500μm from the wound margin. Although a minor activity, mitosis of neurons in mature brains may be a real phenomenon, reflecting neuronal plasticity in response to injury, which may be utilized for the enhancement of neural regeneration.