辛伐他汀预处理对大鼠心肌缺血再灌注的影响

Influence of pretreatment with simvastatin on the reperfusion in rats with myocardial ischemia

目的:观察辛伐他汀预处理对大鼠急性心肌缺血的影响,探讨其对心肌的保护机制。方法:40只雄性SD大鼠随机分为3组,假手术组(12只),缺血再灌注组(14只)用等量生理盐水灌胃,辛伐他汀预处理组(14只)用辛伐他汀20 mg/(kg.d),溶于生理盐水中灌胃,每组再平分为2组,分别再灌注30,90 min。所有动物均灌胃14 d,1次/d,第15天建立大鼠心肌缺血再灌注模型,观察大鼠心肌缺血再灌注后心肌组织中髓过氧化物酶、肿瘤坏死因子-α、白细胞介素-4的动态变化。结果:预处理组心肌组织髓过氧化物酶、肿瘤坏死因子-α、白细胞介素-4水平在再灌注30,90 min各时段均低于缺血再灌注组(P<0.05)。结论:辛伐他汀通过改善内皮功能和抗炎作用对缺血再灌注心肌损伤起保护作用。

Objective To explore the protective effects of pretreatment of simvastatin on acute myocardial ischemia in rats. Methods Forty male SD rats were randomly divided into three groups： sham group（n： 12）, ischemia-reperfusion group（n= 14）, and simvastatin group（n=14）. Rats received simvastatin and saline gastric gavage once a day, totally for 14 days. And on the 15th day, the ischemic-reperfused models were established. Each group was divided into 2 subgroups 30-rain and 90-rain reperfusion groups. The levels of myocardial myeloperoxidase, tumor necrosis factor α and interlukin 4 were measured. Results The levels of myocardial myocardial myeloperoxidase, tumor necrosis faetor-α and interlukin 4 were lower after 30 and 90 minutes reperfusion in simvastatin group than those in ischemic-reperfused group （P〈0.05）. Conclusion Pretreatment with simvastatin has a protective effect on ischemie-reperfused myocardium by improving endothelial function and antiinflammatory effect.