α-硫辛酸对染铅幼年大鼠骨代谢的影响

Effects of α-lipoic acid on bone metabolism in lead-poisoned juvenile rats

目的探讨α-硫辛酸对染铅大鼠骨代谢的影响。方法将31只SD大鼠随机分为2组,空白对照组8只和铅染毒组(230 mg/L的醋酸铅灌胃)23只,40 d后各组取3只大鼠测血铅和骨铅,将已染铅大鼠随机分为4组,即铅对照组、3个α-硫辛酸治疗组[30、60、100 mg/(kg.d)]每组5只。α-硫辛酸治疗80 d后处死动物,测定血铅、骨铅,骨钙素放免分析法测含量,碱性磷酸酶免疫组化法检测表达。结果①各α-硫辛酸治疗组大鼠血铅、骨铅水平均低于铅对照组,差异有统计学意义(P<0.05)。②α-硫辛酸100 mg治疗组大鼠骨钙素含量、碱性磷酸酶表达与铅对照组比较,差异有统计学意义(P<0.05)。结论α-硫辛酸能够减轻铅对骨代谢的毒性作用。

Objective To determine the effects of α-lipoic acid on bone metabolism in lead-poisoned rats.Methods Totally 31 SD rats were randomly divided into two groups,8 rats in blank control group and 23 in lead group that received gastric lavage with lead acetate（230 mg/L）.After 40 days,3 rats from each group were taken for the measurement of lead in the blood and bone.The rats in lead group were then randomly divided into four groups： lead control group and three lead groups with different concentrations of α-lipoic acid [30,60 and 100 mg/（kg·d）],with 5 rats in each.At the end of the experiment（80 d）,the rats were killed and the samples of whole blood,serum and bone were collected to detect osteocalcin content with radioimmunoassay and alkaline phosphatase expression with immunohistochemistry staining.Results ① Blood and bone lead contents in each α-lipoic treatment group were significantly lower than those in lead control group（P〈0.05）.② After treatment with α-lipoic acid of 100 mg,osteocalcin content and alkaline phosphatase expression were significantly different from those in lead control group（P〈0.05）.Conclusion α-lipoic acid can alleviate lead toxicity in bone metabolism.