survivin蛋白及其mRNA在子痫前期患者胎盘中的表达

Expressions of survivin protein and survivin mRNA in placenta with pre-eclampsia

目的探讨survivin蛋白及其mRNA的表达与子痫前期的关系。方法采用免疫组化SP法和实时荧光定量PCR(FQ-PCR)技术,检测20例轻度子痫前期、25例重度子痫前期和20例正常晚孕妇女胎盘组织中survivin蛋白及其mRNA的表达情况。结果 survivin蛋白主要位于绒毛小叶的合体滋养细胞及细胞滋养细胞,在子痫前期胎盘中的表达较正常晚孕妇女明显降低,且重度子痫前期的表达明显低于轻度;survivin mRNA在子痫前期胎盘中的表达也明显低于正常晚孕,重度明显低于轻度。结论胎盘组织中survivin蛋白及其mRNA的异常表达可能在子痫前期的发生中起着重要的作用。

Objective To detect the expressions of survivin protein and survivin mRNA in placenta of women with pre-eclampsia and normal pregnancy.Methods The expressions of survivin protein and survivin mRNA collected from placenta of 45 pre-eclampsia patients（20 cases of mild pre-eclampsia and 25 cases of severe pre-eclampsia） and 20 normal pregnant women were determined by immunohistochemistry.The image analysis technique was employed to analyze survivin protein.Real-time FQ-PCR was used to detect the placental expression of survivin mRNA.Results survivin protein was found mostly in syncytiotrophoblasts and cytotrophoblasts,and it was significantly lower in placenta of pre-eclampsia than that in normal placenta,and significantly lower in severe pre-eclampsia than in mild pre-eclampsia.survivin mRNA was significantly lower in pre-eclampsia than in normal placenta and significantly lower in severe pre-eclampsia than in mild pre-eclampsia.Conclusion The abnormal expressions of survivin protein and mRNA in placental tissues may play an important role in the pathogenesis of pre-eclampsia.