HPLC-ELSD法测定桔梗中3种桔梗皂苷的含量

HPLC-ELSD for determining the content of three platycodins in Radix platycodoi

目的建立测定桔梗药材中桔梗皂苷D、D3、E含量的方法。方法采用高效液相色谱-蒸发光激光闪射检测器（HPLC-ELSD）法,Hypersil C18柱（5μm,4.6 mm×150 mm）,以乙腈-水为流动相梯度洗脱,流速1.0 mL/min,ELSD漂移管温度113℃,载气（N2）流速3.0 L/min。结果桔梗皂苷D、D3、E的线性范围分别为13.78~275.6μg/mL（r=0.999 5）、8.40~168.0μg/mL（r=0.999 7）、12.02~240.4μg/mL（r=0.999 6）,平均回收率（n=5）为98.3%、99.4%和101.3%。结论本法操作简便,结果准确可靠,可用于桔梗皂苷D、D3、E含量的测定。

Objective To establish a quantitative method to determine the content of platycodin D,D3 and E in Radix platycodi.Methods High performance liquid chromatagraphy-evaprorative light scattering dector（HPLC-ELSD） method was adopted.Platycodin D,D3 and E were separated by C18（5 μm,4.6 mm×150 mm） with acetonitrile-water as mobile phase in a gradient program;flow rate was 1.0 mL/min,the temperature of drift tube was set at 113 ℃,and the gas flow（N2） was set at 3.0 L/min.Results The linear ranges of platycodin D,D3 and E were 13.78-275.6 μg/mL（r=0.999 5）,8.40-168.0 μg/mL（r=0.999 7） and 12.02-240.4 μg/mL（r=0.999 6）,respectively.The average recoveries（n=5） were 98.3%,99.4% and 101.3%,respectively.Conclusion The method is convenient,accurate and reliable,and can be used for the determination of platycodin D,D3 and E in Radix platycodoi.