当归多糖照射前后给药对放射损伤小鼠造血功能恢复的比较研究

Compared study of hematopoietic recovery of angelica polysaccharides dosaged pre-irradiation and post irradiation on radiation injured mice

目的:研究和比较当归多糖(Adhesion molecule,APS)2种不同给药方式对放射损伤小鼠造血功能的修复作用。方法:外周血WBC、RBC、PLT及BMNC计数;流式细胞术检测APS对放射损伤小鼠Sca-1+BMNC粘附分子CD44和CD49d表达、BMNC细胞周期的影响;MTT法测定APS对放射损伤小鼠骨髓细胞增殖活性的影响。结果:与正常组比较,各NS组外周血WBC、RBC、PLT及BMNC计数明显减少,Sca-1+BMNCCD44、CD49d表达、骨髓细胞增殖活性明显下降,BMNCG0/G1期细胞比例显著增加。各APS预处理组能在更早期促进外周血各系和BMNC数目恢复、更早促进Sca-1+BMNC表面CD44和CD49d的表达下降使放射损伤小鼠造血功能较同时间点、同等剂量APS治疗组恢复快。各APS预处理组骨髓细胞增殖活性、BMNCG0/G1期细胞比例较同时间点、同等剂量APS治疗组差异无统计学意义(P>0.05)。结论:APS对放射损伤小鼠造血功能具有修复作用,初步观察APS预处理组优于APS治疗组。

Objective：To investigate the effects of Angelica polysaccharides on hematopoietic type of acute radiation sickness in mice.Methods：Techniques of peripheral blood cells and BMNC count were made;flow cytometry detected the expression of CD49d, CD44 on Sca-1＋BMNC and the cell cycle on BMNC, the proliferation of BMNC were evaluated by MTT assay.Results：Ascompared with those in control group,the numbers of the peripheral leukocyte,erythrocyte,platelet and BMNC in NS （normal saline） group decreased significantly;the expression of CD49d,CD44 on Sca-1＋BMNC and the proliferation of BMNC declined;and the percentage of G0/G1 phases significantly increased.APS pretreatment could promote peripheral blood cells and BMNC numbers to recover, decrease the expression of CD49d,CD44 on Sca-1＋BMNC in earlier period to make the hematopoietic function of radiation injured mice in pretreated groups to recover more quickly,as compared with APS treated groups receiving the same dose at the same time.The proliferation of BMNC and the percentage of G0/G1 phases in APS pretreated groups had no significant differences from those in APS treated groups receiving the same dose at the same time （P0.05）.Conclusion：The results support our hypothesis that APS can be used to treat the hematopoietic type of acute radiation sickness.APS pretreated groups had better results than APS treated groups in initial observation.