甲基化抑制剂5-氮杂胞苷对HL-60细胞增殖及凋亡的影响

5-Aza-cytidine inhibit proliferation of human promyelocytic leukemia cells (HL60) by inducing apoptosis

目的:观察5-氮杂胞苷对髓系白血病细胞系HL-60的增殖抑制作用,探讨其对细胞周期、细胞凋亡的影响。方法:常规方法培养HL-60细胞,加入不同浓度的5-氮杂胞苷,以MTT法测定对HL-60的抑制作用,计算细胞生长抑制率;流式细胞术检测小剂量5-氮杂胞苷处理HL-60细胞后48h的细胞凋亡率及细胞周期分布特点。结果:不同浓度5-氮杂胞苷均可以不同程度的抑制细胞生长,且表现为时间、浓度依赖性。在小剂量浓度5-氮杂胞苷处理HL-60细胞48h后,随着5-氮杂胞苷浓度的增加,凋亡细胞百分比逐渐增多(P<0.05);G1期细胞逐渐增多,S期细胞减少,G2/M期细胞相对增多,细胞阻滞在G1期。结论:5-氮杂胞苷对髓系白细胞细胞系HL-60的生长抑制表现为浓度和时间依赖性,一定剂量的5-氮杂胞苷诱导细胞凋亡,使细胞阻滞在G1期。

Objevtive：This study was aimed to investigate the effect of 5-aza-cytidine on the proliferation,cell cycles distribution profile and apoptosis of human promyelocytic cell line HL60.Methods： The human promyelocytic cell line HL-60 was cultured in RPMI 1640 medium.3-（4,5-Dimethylthiazol-2-y）l-2,5-diphenylte trazolium（MTT）reduction by cells was used to assess drug-induced cell growth inhibition.Cells were treated with 0.25,0.5,1,2.5μmol/L of freshly prepared 5-aza-cytidine for 48 hours before they were harvested for analyzing distribution of cell cycle phases and apoptosis by flow cytometer.Results：5-aza-cytidine inhibited HL60 cell proliferation in a time-and concentration-dependent manner.Apoptosis was induced as detected by flow cytometry.Determination of the cell cycle distribution by flow cytometry revealed an accumulation of cells in G1 phase.Conclusion：DNA methylation inhibitor 5-aza-cytidine has effects on proliferation of human promyelocytic cell line HL60.Possible mechanism of this inhibition is induction of apoptosis.