通痹合剂对雄性大鼠抗生育作用及其可逆性的研究

Anti-fertility effect of Tongbi Composition and its reversibility in male rats

目的:观察临床最大剂量的通痹合剂对雄性大鼠的抗生育效应及其可逆性。方法:36只成熟雄性SD大鼠,随机分成2组,每组18只:对照组喂服生理盐水10ml/(kg·d),给药组喂服通痹合剂10g/(kg·d),连续60d后,随机处死半数大鼠,取材。余下大鼠恢复饲养72d后,处死取材。检测大鼠睾丸系数、睾丸体积、精子浓度及活力。光镜观察睾丸组织的病理变化。结果:给药60d后,与对照组比较,给药组睾丸系数、睾丸体积、精子浓度均无统计学差异(P>0.05),精子活动率下降为零;而停药72d后,精子活动力恢复,运动活跃。光镜下给药组睾丸组织几乎无病理损伤。结论:短期应用临床最大剂量的通痹合剂导致的雄性抗生育作用具有可逆性。

Objective ：To study the anti-fertility effect of maximum-dose Tongbi Composition and its reversibility in male rats. Methods ：Thirty-six male SD rats were equally randomized into a control group and a medication group,the former given normal saline at 10 ml/（kg·d）,while the latter treated with Tongbi Composition at 10 g/（kg·d）,both for 60 days. Half the rats of each group were sacrificed randomly at the cessation of treatment,and the rest killed at 72 days after it. The relative testis weight,testis volume,sperm concentration and sperm motility were measured,and the pathological changes in the testicular tissue observed under the optical microscope. Results ：After 60 days of treatment,no statistically significant differences were found between the two groups in the relative testis weight,testis volume and sperm concentration （P 0.05）,and the sperm motility of the medication group dropped to zero,but it was restored to normal at 72 days after drug withdrawal. Almost no lesions were observed in the testis tissue of the medication group. Conclusion ：The short-term use of Tongbi Composition at the maximum clinical dose has an obvious anti-fertility effect,but it is reversible.