驱虫斑鸠菊注射液联合NB-UVB照射对小鼠表皮c-KIT和PAR-2蛋白表达的影响

Effects of Vernoniaanthelmintica Willd Combined NB-UVB Irradiation on the Expression of c-KIT and PAR-2 Protein in Mice Epidermis

目的探讨驱虫斑鸠菊注射液联合窄谱中波紫外线(NB-UVB)照射临床治疗白癜风有效的可能机制。方法 40只小鼠随机分为4组即对照组(C),NB-UVB照射组(UV),驱虫斑鸠菊注射液组(VW),驱虫斑鸠菊注射液+NB-UVB组(VW+UV)。C组给予生理盐水腹腔注射,UV组给予生理盐水腹腔注射后NB-UVB照射,VW组给予驱虫斑鸠菊注射液腹腔注射,VW+UV组给予驱虫斑鸠菊注射液腹腔注射后进行NB-UVB照射,各1次/d,14天后取背部皮肤进行组织检查。采用免疫组化检测小鼠表皮中干细胞因子受体(c-KIT)和蛋白酶激活受体-2(PAR-2)表达量的变化。结果与C组相比,UV,VW,VW+UV组的c-KIT和PAR-2阳性细胞数均有增加,差异有统计学意义(P<0.05);而VW+UV组c-KIT和PAR-2的表达水平最强,与UV、VW组差异有统计学意义(P<0.05)。结论驱虫斑鸠菊注射液联合窄谱中波紫外线照射临床治疗白癜风有效的机理可能与表皮c-kit和par-2蛋白表达增强有关,从而促进了色素沉着的发生。

Objective To explore the possible mechanism of extracts of vernoniaanthelmintica willd（vw） combined NB- UVB radiation in the treatment of vitiligo. Methods Forty BALB/C mouse were randomized into four groups comprising control group （ C）, ultraviolet exposed group （ UV）, vernoniaanthelmintica willd injection group （VW） and vernoniaanthelmintica willd injection and ultraviolet exposed group （VW ＋ UV）. Mouse of normal control group were injected with normal saline ; mouse of UV group were irradiated with NB-UVB ra- diation ; VW group were injected with vernoniaanthelmintica willd injection and VW ＋ UV group were treated with vernoniaanthelmintica willd injection and ultraviolet exposed. A dose of VW were 5mg and dose of NB were 1J/cm^2 were given every day for 2 weeks. After NB-UVB radiation, the skin specimens were obtained. Immunohistochemistry method was used to detect c-KIT and PAR-2 protein expression in these specimens. Results Compared with the C group, there was a significant increase in the positive rates of c-kit and PAR- 2 expression in UV,VW and VW ＋ UV group（P 〈0. 05）, meanwhile c-kit and PAR-2 expression levels in the VW ＋ UV group were significantly higher than those in UV and VW group（ P 〈0.05 ）. C-kit 和 PAR-2 expression levels in the VW and VW ＋ UV groups had no significant difference （P 〉 0.05 ）. Conclusion Our results suggest that the efficacy of Vernoniaanthelmintica willd combined NB-UVB irradiation in the treatment of vitiligo may be related to its ability to enhance c-kit and PAR-2 expression levels, accordingly promoting the production of pigment.