南京地区西花蓟马发生调查及其分子检测

Survey in Nanjing and the PCR diagnosis of Frankliniella occidentalis

以西花蓟马和烟蓟马的线粒体DNA的COI基因设计特异性引物,建立了西花蓟马PCR快速检测方法,该方法可以检测1/120单头成虫,对各种虫态均可进行特异性检测。对南京54种植物花器中蓟马种类进行了调查,通过形态特征和线粒体DNA的COI基因分子检测,均证明西花蓟马已在其中25种植物上发生,西花蓟马种群数量达蓟马总量20%的有11种植物,超过30%的有5种。

Primers were designed based on the COI gene of mtDNA in Frankliniella occidentalis （Pergander） and Thrips tobaci Lindeman,and the PCR diagnosis was developed for F.occidentalis.The reliability of the diagnostic test were validated by performing with different stages of the pest,even 1 /120 of the DNA from singal adult.The survey of flower thrips host plants was conducted among 54 plant varieties in Nanjing,and the results from morphological observation by means of optical and electronic scanning microscope,and from PCR diognostic test,proved that the alien invasive insect—western flower thrips occurred in 25 out of 54 plant varieties in Nanjing,and indicated in 11 varieties the population density of western flower thrips were over 20% of the total thrips,and in 5 varieties the proprotion was even higher than 30%.