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天然产物Genistein抑制人胃癌细胞的磷酸化蛋白组学研究

The Phospho-Proteomics Research of Natural Product Genistein Inhibiting Human Gastric Cancer Cell
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摘要 研究天然产物genistein调控的磷酸化蛋白质分子,探讨其抑癌的作用机理。文章用40μmol/Lgenistein作用于人胃癌细胞SGC-7901后,采用二氧化钛富集磷酸化肽段,利用LTQ-orbitrap质谱进行磷酸化肽段和磷酸化位点鉴定,结果显示在人胃癌细胞SGC-7901和用genistein处理的胃癌细胞SGC-7901共出现45个差异磷酸化蛋白,通过对这些差异磷酸化蛋白质的比较,从蛋白组学研究方面揭示天然产物genistein作用机理。 The research of phosphorylation protein molecules regulated by natural products genistein was to explore its role in the tumor suppressor mechanism. In this paper, 40umol/L genistein was applied on human gastric cancer cell SGC- 7901 following the introduction of phospho-peptide enrichment kit to enrichment of phosphorylated peptides. Phosphorylated peptides and phosphorylation sites were identified using LTQ- orbitrap mass spectrometry. Results showed that there were 45 different phosphorylated proteins between normal human gastric cancer cell SGC- 7901 and the genisteintreatedgastric cancer cell SGC- 7901. Compared with the different phosphorylated proteins, it revealed the mechanism of natural products genistein in the proteomics research.
作者 贾红玲 徐振 何桂卫
机构地区 暨南大学生命与健康工程研究院
出处 《药物生物技术》 CAS CSCD 2010年第4期297-303,共7页 Pharmaceutical Biotechnology
基金 暨南大学211工程"生物材料与组织工程"青年基金项目50621030
关键词 胃癌 三羟基异黄酮 磷酸化 二氧化钛富集 质谱分析 Gastric cancer, Genistein, Phosphorylated, TiO2 enrichment, Mass spectrometry
分类号 Q51 [生物学—生物化学] Q55 [生物学—生物化学]
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参考文献17

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药物生物技术
2010年 第4期

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