多重PCR技术在呼吸道暴发疫情中的应用

Application of multiple PCR technology in respiratory outbreak

目的:用有效的方法快速检测呼吸道暴发疫情中的病毒病原学感染现况,探讨病毒活动状况及流行规律。方法:收集宜昌地区2009年全年报告的所有呼吸道疫情标本,先采用RT-PCR方法检测季节性流感病毒和新甲型H1N1流感病毒,排除流感的阴性疫情标本再采用多重RT-PCR法,同时检测腺病毒、间质肺病毒、冠状病毒229E/NL63、副流感病毒1、2、3型;甲、乙型流感病毒、呼吸道合胞病毒A\B型、鼻病毒、冠状病毒OC43,共12种常见呼吸道病毒。结果:2009年本市报告的29起呼吸道疫情中,流感疫情24起,约占总疫情数的82.8%,非流感疫情中呼吸道合胞病毒和间质肺病毒检出率最高,其次为副流感病毒及冠状病毒。结论:流感病毒、呼吸道合胞病毒、间质肺病毒、冠状病毒和副流感病毒为宜昌地区呼吸道暴发疫情中病毒感染的主要致病原。多重PCR技术在疫情的快速检测方面准确高效,能尽快明确疫情感染的病原体,为防控争取到宝贵时间;从而对指导爆发疫情的处理发挥了重要作用。

Objective：To study the virus etiology of respiratory outbreak and explore virus activity and prevalent regularity.Methods：We detect seasonal flu virus and Pandemic（H1N1） 2009 by RT-PCR,then we detect 12 kinds of common respiratory viruses on the negative specimens excluded influenza by multiple RT–PCR,they are adenovirus,metapneumovirus,coronavir229E/NL63,parainfluenza virus 1,2,3,influenza virusA,B,respiratory syncytial virusA,B,rhinovirusA and coronavirusOC43.Results： 29 respiratory outbreak were reported in 2009,among them,the influenza epidemic was 24,it was 82.8%of all the total epidemic.RsV and Mpv infection Were the most frequent in negative influenza outbreak.PIV and Coronavirus were second.Conclusion：Flu,Rsv,Mpv,Coronavirus and Piv were the main pathogens leading to the respiratory outbreak in Yichang.Multiple PCR technology is accurate and efficient in rapid detection of respiratory outbreak,and it plays an important role in guiding the treatment of epidemic outbreak.