HPLC法测定酚麻美敏片中3种组分的含量

HPLC determination of three components in compound acetaminophen caplets tablets

目的:采用高效液相色谱法,对酚麻美敏片中盐酸伪麻黄碱、马来酸氯苯那敏和氢溴酸右美沙芬进行分离和含量测定。方法:采用ZORBAX300-SCX柱(4.6mm×250mm,5μm),流动相为乙腈-0.02mol·L-1磷酸二氢钾溶液(55∶45),流速:1.0mL.min-1,检测波长263nm,柱温:28℃。结果:盐酸麻黄碱、马来酸氯苯那敏和氢溴酸右美沙芬浓度分别在0.06～0.6mg·mL-1(r=0.9992),0.04～0.4mg·mL-1(r=0.9998)和0.032～0.32mg·mL-1(r=0.9992)范围内呈良好的线性关系;平均回收率分别为94.2%～102.2%,95.3%～101.1%和98.5%～102.2%。结论:本法快速、简便,重复性好。

Objective：To establish a method for the determination of three components in acetaminophen compound caplets tablets by HPLC.Method：The ZORBAX 300-SCX column （4.6 mm×250 mm,5 μm） was used,mobile phase consisted of acetonitrile-0.02 mol·L-1potassium dihydrogen phosphate solution （55：45） at a flow rate of 1.0 mL·min-1,detection wavelength being 263 nm and temperature being 28 ℃.Result：The standard curves of pseudoephedrine hydrochloride,chlorpheniramine maleate and dextromethorphan hydrobromide showed good linearity over the range of 0.06-0.6 mg·mL-1 （r=0.9992）,0.04-0.4 mg·mL-1（r=0.9998） and 0.032-0.32 mg·mL-1（r=0.9992）,respectively.The average recoveries of the method were 94.2%-102.2%,95.3%-101.1% and 98.5%-102.2%.Conclusion：The method is simple,rapid and reproducible.