高效液相色谱法蒸发光散射检测器测定泽桂癃爽片中熊果酸的含量

HPLC-ELSD determination of ursolic acid in Zegui Longshuang tablets

目的:建立HPLC-ELSD法测定泽桂癃爽片中熊果酸的含量。方法:色谱柱:KromasilC18(150mm×4.6mm5μm);流动相:乙腈-甲醇-水(0.5%醋酸铵)=(64∶20∶16);流速:1mL.min-1;ELSD-2000检测器;漂移管温度:76℃;气体:氮气,流量2.0L.min-1。结果:熊果酸在0.26～1.30μg范围内线性关系良好(r=0.9998)。平均回收率为:98.0%;RSD为:0.4%(n=5)。结论:本法快速,简便,准确,重复性良好。

Objective：To develop an HPLC-ELSD method for detemining the contents of ursolic acid in Zegui Longshuang tablets.Methods：The analytical column was Kromasil C18（150 mm×4.6 mm,5 μm）;The mobile phase was acetonitrile-methanol-water（0.5% acetic amine）（64：20：16）at flow rate of 1 mL·min-1;An eweaporative light-scattering detector（ELSD）Model 2000,the drift tube temperature：76 ℃.The gas flow rate：2.0 L·min-1.Results：In the scope of 0.26-1.30 μg the contents of Ursolic Acid had good linear relationship with their peak area（r=0.9998）,The average recoveries were 98.0%;RSD=0.4%（n=5）.Conclusion：The results showed that the method was rapid,simple,excellent precision.