花刺柳珊瑚共生真菌Penicillium sp. gx wz406的次生代谢产物研究

Secondary metabolites from Penicillium sp.gxwz406 symbiotic with the gorgonian Echinogorgia flora

目的从珊瑚共生真菌中获得抗肿瘤药物先导化合物。方法通过集成化学与细胞毒性的筛选方法筛选目标菌株,采用现代色谱和波谱的方法分离鉴定目标菌株的活性代谢产物,通过化学沟通鉴定化合物的绝对立体构型。结果和结论从采自广西涠洲岛的花刺柳珊瑚(Echinogorgia floraNutting)中筛选获得了1株具有细胞毒活性的青霉菌(Penicillium sp.)gxwz406,从其发酵产物中分离鉴定了5个化合物:S(-)-2-(2-hydroxypropanamido)benzamide(1)、R(+)-chrysogine(2)2、-pyruvylaminobenzamide(3)、nicotinamide(4)和2',3'-dihydrosorbicillin(5),并首次确定了化合物1的绝对构型。

Objective To search for structurally novel and biologically active compounds from marine-derived fungi associated with the coral.Methods Integrated chemical and cytotoxic method was used to screen the working strains.Compounds were isolated by chromatographic process,and their structures were identified by spectroscopic analyses and chemical transformation.Results and Conclusion A cytotoxic fungal strain gxwz406 identified as Penicillium sp.was isolated from the gorgonian Echinogorgia flora Nutting from Weizhou Island,Guangxi province.Five compounds were isolated from the fermentation broth of Penicillium sp.gxwz406.Their structures were elucidated as S（-）-2-（2-hydroxypropanamido）benzamide（1）,R（＋）-chrysogine（2）,2-pyruvylaminobenzamide（3）,nicotinamide（4）,and 2＇,3＇-dihydrosorbicillin（5）,respectively.And the absolute configuration of 1 was determined for the first time in this paper.