不同年龄组健康儿童外周血淋巴细胞亚群的变化

Changes of Lymphocyte Subsets in Healthy Children within Different Age Groups

目的了解不同年龄组儿童淋巴细胞亚群的变化。方法将来自本院儿科门诊进行体检的年龄为6个月~13岁的健康儿童77例分为4个不同年龄组：婴儿组22例,年龄（7.09±0.52）个月;幼儿组21例,年龄（2.61±0.49）岁;学龄前组14例,年龄（4.64±0.92）岁;学龄组20例,年龄（9.80±2.11）岁。采用流式细胞仪对其外周静脉血进行淋巴细胞亚群百分率检测,应用SPSS16.0软件进行数据处理。结果 CD3＋和CD8＋T淋巴细胞百分率随年龄增长而升高;B淋巴细胞百分率随年龄增长而降低,由婴儿组的（21.77±8.37）%降至学龄组的（14.84±3.55）%（P〈0.001）。CD4/CD8比值也随年龄增大呈减少的趋势,但仅婴儿组和学龄组间差异有统计学意义（P〈0.05）。各年龄组间CD4及自然杀伤细胞百分率比较差异均无统计学意义（Pa〉0.05）。结论年龄对儿童外周血淋巴细胞百分率有一定影响,但2岁以上儿童除CD8外,其他各项指标差异均无统计学意义。

Objective To study the changes of lymphocyte subsets in healthy children within different age groups.Methods The total of 77 healthy children aged from 6 months to 13 years old receiving health examination in pediatric outpatient department were divided into 4 groups.The groups were as follows,infant group（22 cases） with average age（7.09±0.52） months,kindergarten children group（21 cases） with average age（2.61±0.49） years old,preschool children group（14 cases） with average age（4.64±0.92） years old and school children group（20 cases） with average age（9.80±2.11） years old.The percentage of lymphocyte subsets were analyzed by means of flow cytometry.SPSS 16.0 software was used to analyze the data.Results The study demonstrated that percentage of lymphocytes with CD3 increased with age and so was the lymphocyte with CD8.Whereas the B cells percentage and the ratio of CD4/CD8 decreased with age and the difference was found between infant group[（21.77±8.37）%] and school age group[（14.84±3.55）%]（P0.001）.There was no significantly statistic difference in CD4 and natural killer percentages between any age group（P0.05）.Conclusions Age has some effect on average percentage of lymphocyte subsets especially between infants and older children.However,except for CD8,there are no significantly statistic differences in children over 2 years old.