应用免疫透射比浊法测定免疫球蛋白方法学的建立

Measurement of Immunoglobulin and Complement by Immunoturbidimetric Assay

一 为建立免疫球蛋白及补体血清浓度的免疫透射检测方法。利用伊利康生物技术有限公司特种蛋白试剂在Olympus Au560上建立分析方法。此方法平均收回率为98.2％,批内变异系数≤3.0％,批间<5.0％,用卫生部临检中心提供的质控血清做比较,所有结果均符合此标准。免疫比浊测定具有灵敏、准确、稳定、特异、干扰少的特点,可以做为免疫球蛋白血清浓度的非放射性常规方法。

It is to establish immunoturbidimetric assay to measure immunoglobulin and complement serum concentration methods. By special protein reagents of Yi Li Kang biological technology Co,LTD. A method of immunoturbidimetric assay was established in Olympus Au560 analyzer. Immunoturbidimetic assay has a average of 98. 2%. At the most within run cv of 3. 0%for intra-run and 5. 0% for inter-run. The results of our methods are comparable with reference serum provided by clinical examine centre Health Department. All of results fulfiled the quarlity control performernce criteria. Immunoturbidimetric assay is a sensitive acurate. stable. no sig-nificent interference and spelific menthod. It is a qualified comdidate for immunoglobin and complement serun Level detection nonradioactive Routine.