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显微操作注射针对牛ICSI效率的影响 被引量:2

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摘要 借助显微操作仪将牛精子注入经体外成熟22h的牛卵母细胞胞质内,并对注射卵进行体外培养,观察其存活及发育情况,探讨了显微操作注射针对牛ICSI胚胎体外发育的影响,研究结果表明,用外径为3~5μm的注射针进行显微注射的注射卵存活率、分裂率和囊胚率分别为84.80%、57.55%和22.64%,均显著高于8~10μm的注射针处理组(76.56%,45.92%,14.29%)(P〈0.05)。
作者 张营霞
机构地区 山东省枣庄学院
出处 《江苏农业科学》 CSCD 北大核心 2010年第4期211-211,214,共2页 Jiangsu Agricultural Sciences
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参考文献4

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同被引文献17

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  • 9黄仲英,李尚为,马黔红,樊伟,李蕾,李小红,胡寒.降低聚乙烯吡咯烷酮浓度对卵胞浆内单精子显微注射效果的影响[J].实用医学杂志,2009,25(1):68-70. 被引量:4
  • 10邓利,曾明,匡延平,邬玲仟,吕祁峰.小鼠卵子胞膜在显微穿刺中存活的膜特性因素研究[J].生殖与避孕,2011,31(12):791-796. 被引量:3

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