健康成人和放射工作者体细胞HPRT基因位点突变率的检测和比较

Detection and comparison of mutant frequency of somatic HPRT locus between healthy adults and radiation workers

目的为了解健康成人和放射工作者体细胞ＨＰＲＴ基因位点突变率。方法应用人外周血淋巴细胞抗６－ＴＧ分析技术检测了３０例健康成人和３０例放射工作者体细胞ＨＰＲＴ位点突变频率。结果健康成人ＨＰＲＴ基因位点突变频率均值为０１６２８×１０－３，变化范围为０１３００×１０－４～０２９０３×１０－３，放射工作ＨＰＲＴ基因位点的突变频率均值为０２２２５×１０－３，变化范围为０３３７×１０－４～０４７８×１０－３。ｔ检验Ｐ＜００５，两者差异显著。从检测结果亦可看出，随着放射工龄的延长，ＨＰＲＴ基因位点突变频率也随之增加。结论体细胞ＨＰＲＴ基因位点突变频率可作为各种有害因素对机体遗传损伤的生物标识物之一。

Objective In order to study mutant frequencies of somatic HPRT locus between healthy adults and radiation workers. Methods Mutant frequencies of somatic HPRT locus in peripheral blood lymphocytes of 30 healthy adults and 30 radiation workers were detected by using 6 thioguanine resistant(TG r)assay. Results The results showed that the TG r mutant frequency in the healthy adults was 0 1628×10 -3 ranged from 0 1300×10 -4 ～0 2903×10 -3 . It was 0 2225×10 -3 ,ranged from 0 337×10 -4 ～0 478×10 -3 in the radiation workers. A significant difference was shown between healthy adults and radiation workers. The TG r mutant frequency also appeared to be increased with the length of radiation service. Conclusion The mutant frequency at HPRT locus can be served as one of the biomarkers for detecting genetic damage of human exposure to environmental and occupational mutagens.