五肽胃泌素及丙谷胺对人结肠癌细胞增殖的影响

The Effect of Pentagastrin and Proglumide on the Proliferation of Human Colon Adenocarcinoma Cells

目的：探讨五肽胃泌素（ｐｅｎｔａｇａｓｔｒｉｎＰＧ）及丙谷胺（ｐｒｏｇｌｕｍｉｄｅＰＧＬ）对体外培养的人结肠癌细胞株ＨＴ２９增殖的影响。方法：用ＭＴＴ法测定细胞生长曲线，用流式细胞仪分析细胞周期变化。结果：ＰＧ能明显刺激ＨＴ２９细胞增殖，２．５×１０－６ｍｏｌ／ＬＰＧ使细胞倍增时间缩短５小时，提高细胞增殖指数（Ｐ＜０．０１）。ＰＧＬ能抑制ＰＧ的促增殖作用，单独使用能抑制ＨＴ２９细胞增殖，４ｍＭＰＧＬ能使ＨＴ２９细胞倍增时间延长１１小时，并降低增殖指数（Ｐ＜０．０１）。结论：胃泌素可能在结肠癌的生长中具有调节作用，胃泌素受体拮抗剂可能在结肠癌的治疗中具有重要作用。

Objective:To study the effect of pentagastrin (PG)and proglumide (PGL)on the proliferation of human colon adenocarcinoma cell line HT29 cultured in vitro.Method:The proliferation of HT29 cells was determined by MTT colorimetry.The cell cycles was analyzed by flow cytometry.Results:PG could significantly promote the proliferation of HT29 cells.When concentration of PG were 2 5×10 -6 mol/L The time of multiple proliferation of HT29 cells were decreased 5 hours and the cell proliferative index(PI) was increased(in the presence of 2 5×10 -6 mPG ( P <0 01).PGL could significantly inhibite the proliferation of HT29 cells alone and block the stimulation effect of PG on HT29 cells.The time of multiple proliferation of HT29 cells were increased 11 hours and the PI was decreased in the presence of 4 mMol/L PGL( P <0 01).Conclusion:Gastrin may be as a grouth regulator in colon carcinoma.Gastrin receptor antagonists might have clinical value in treating human colon cancer in future.