饮食诱导肥胖和肥胖抵抗大鼠脂联素水平研究

ADIPONECTIN LEVELS IN DIETARY INDUCED OBESITY AND OBESITY-RESISTANT RATS

目的探讨高脂饲料对大鼠内脏脂肪中脂联素表达和血清中脂联素水平的影响及大鼠肥胖易感性差异的机制。方法 80只雄性SD大鼠,按体重随机分为两组:对照组(n=10),基础饲料喂养20w;高脂组(n=70),高脂饲料喂养12w。第12w末,根据体重将高脂组分为饮食诱导肥胖(DIO)和肥胖抵抗(DIO-R)组,继续喂养8w,观察各组大鼠体重、内脏脂肪湿重、体脂比、热能摄入量及能量利用率的差异;用Real Time PCR法测定内脏脂肪脂联素mRNA的表达水平;ELISA法测定血清脂联素水平。结果 DIO组体重、内脏脂肪湿重、体脂比、能量利用率显著高于对照组和DIO-R,而DIO-R除能量的摄入量显著低于对照组外,其他与对照组相比未见显著性差异;DIO大鼠内脏脂肪脂联素mRNA的表达水平显著低于对照组和DIO-R;DIO大鼠血清脂联素水平显著低于DIO-R。结论高脂饲料诱导下,不同肥胖易感性大鼠体内脂联素水平有明显差异性,脂联素可能在肥胖形成过程中扮演重要角色。

Objective To study the effect of high-fat diet（HFD） on the expression of adiponectin mRNA,the level of serum adiponectin,and the proper mechanism of different susceptibility to obesity.Method Eighty male Sprague-Dawley（SD） rats were randomly divided into control group（n=10） and high-fat group（n=70）,and fed normal and HFD respectively for 12 w.HFD group was subdivided into obesity（DIO） and obesity-resistant rats（DIO-R） according to body weight,and fed HFD continuously for another 8w.The body weight and food intake were recorded.Wet weight of fat pad and the ratio of fat to body weight were measured at the end of experiment.Adiponectin mRNA in adipose tissue was detected by real time PCR,and serum adiponectin was detected by ELISA.Results The body weights,body fat percent,caloric intake,energy efficiency in DIO rats were higher significantly than those in DIO-R rats,but adiponectin mRNA and serum adiponectin in DIO rats were lower than those in DIO-R rats.Conclusion The adiponectin mRNA expression in adipose tissae and serum adiponectin concentration were decreased in the rats fed high-fat diet and might be closely associated with the development of different susceptibility to obesity.