摘要
目的探讨ｒａｓ癌基因在大肠腺瘤、大肠癌组织中的表达及意义。方法应用免疫组织化学ＬＳＡＢ法，检测５４例大肠癌、１７例大肠腺瘤和３１例正常粘膜中的ｒａｓｐ２１的分布。结果癌组织及腺瘤组织中均有ｒａｓｐ２１过量表达，阳性率分别为５７．４％和２９．４％，均高于正常粘膜（Ｐ＜０．０５），在大肠癌淋巴结转移组ｐ２１阳性表达高于非淋巴结转移组（Ｐ＜０．０５）。结论ｒａｓｐ２１的过量表达与大肠癌的发生及淋巴结转移有关。ｒａｓｐ２１在大肠腺瘤不典型增生上皮中的高表达，可作为早期癌变的参考诊断指标，大肠癌ｐ２１表达阳性率与癌分化程度。
Objective To study the expression of cancer gene product in colorectal adenoma and adenocarcinoma.Method Immunohistochemical staining(LSAB methods) was used to determine the expression of ras p21 in 31 cases normal mucosa,17 cases colorectal adenoma and 54 cases colorectal adenocarcinoma tissue.Result The results showed that the positive rates of ras p21 were 57.4% in adenocarcinoma and 29.4% in adenoma and 3.13% in normal mucosa(P<0.05).The positive rates in group with lymph node metastases was significantly higher than that in those without metastases (P<0.01).Conclusion The over expression of p21 in colorectal carcinoma were related to colorectal carcinogenesis and related to lymph metastases.The positive rates of p21 in carcinoma was no related to the degree of differentiation and no related to the depth of infiltration.
出处
《潍坊医学院学报》
1999年第1期1-2,共2页
Acta Academiae Medicinae Weifang
关键词
大肠癌
癌基因
免疫组织化学
腺瘤
Oncoprotein
Immunohistochemistry
Colorectalcancer
Adenoma
ras p21
