摘要
检测乙型肝炎病毒(HBV)前C基因突变株感染者的细胞免疫水平,探讨细胞免疫与前C基因突变的关系。方法用多聚酶链反应(PCR)结合地高辛标记的寡核苷酸探针杂交技术从60名慢性乙肝患者中筛选出HBV前C基因突变株感染者、HBV野毒株感染者及HBV已清除者各5人,用血源性HBsAg和CD4+T辅助细胞识别的抗原表位HBcAg50-69合成多肽进行特异性淋巴细胞转化实验。结果HBV前C基因突变株感染者对HBsAg的应答与其余各组间差异不显著(P>0.05);对HBcAg50-69合成多肽的应答,与其余各组间差异有高度显著性(P<0.01)。结论HBV前C基因突变株感染者对合成多肽的应答较野毒株感染者及HBV已清除者低。
Objective To study the cellular immunity of chronic hepatitis B patients with precore( pre c) mutation and the relationship between cellular immunity and pre C mutation.Method PCR combining digoxin tagged oligonucleotide probe was used to detect the pre C region of HBV DNA.5 cases patients with wild type HBV infection,5 with pre C mutants infection,5 with anti HBe positive,HBV DNA negative were selected.Result It was found that the CD 4+ T cell response to HBsAg was not significantly different between patients with pre C mutants infection and other groups(P>0.05),However,the CD 4+ T cell response to HBcAg50 69 synthetic peptide in the patients with pre C mutants infection decreased significantly comparing with the patients with wild type HBV infection and patients with anti HBe positive,HBV DNA negative(P<0.01).Conclusion The cellular immunity of chronic hepatitis B patients may relate to HBV pre C mutation.
出处
《潍坊医学院学报》
1999年第1期24-25,共2页
Acta Academiae Medicinae Weifang