通关胶囊对增生前列腺组织细胞外基质的影响及机制研究

Influence of Tongguan Capsule on ECM in BPH patients and its mechanism

目的评价通关胶囊对良性前列腺增生(BPH)患者的增生前列腺细胞外基质(ECM)的影响,并探讨其作用机理。方法选择具备手术指征的BPH病人30例,按就诊顺序随机分为通关胶囊组、非那雄胺组和安慰剂组。服药4周后,行TURP术,留取组织标本。另选正常前列腺标本6例,用免疫组化法结合图像分析系统研究正常组、安慰剂组、通关胶囊组和非那雄胺组前列腺组织纤维连接蛋白(FN)、胶原(CL)、基质金属蛋白酶2(MMP-2)、金属蛋白酶组织抑制因子2(TIMP-2)和模型基质金属蛋白酶1(MT1-MMP)的阳性表达。结果安慰剂组前列腺组织的FN、CL和MT1-MMP的阳性表达较正常组显著增强,差异有统计学意义(P<0.01),MMP-2/TIMP-2差异无统计学意义(P>0.05);通关胶囊组、非那雄胺组与安慰剂组的前列腺组织比较,FN和CL的阳性表达较安慰剂组明显减弱(P<0.01),MT1-MMP和MMP-2/TIMP-2较安慰剂组明显增高(P<0.01)。结论通关胶囊能降低增生前列腺组织ECM成分,避免其沉积,其机理可能与其调节ECM成分及其相关调控因子在前列腺组织中的表达有关。

Objective To evaluate influence of Tongguan Capsule on ECM in prostate tissues of BPH patients and its mechanism. Methods Total of 30 BPH patients who had surgery indications, were randomly divided into Tongguan Capsule group, finasteride group and placebo group. Four weeks after administration, the patients were treated by TURP, and the resected tissues were collected. Expressions of FN, CL, MMP-2, TIMP-2 and MT1-MMP in prostatic tissues of three groups were detected by immunohistochemistry analysis. Results The expressions of FN, CL and MT1-MMP in hyperplastic prostate of placebo group were significantly higher than those of normal group（P〈0. 01）, but no significant change in the expression of MMP-2/TIMP-2 was found （P〉0.05）. The expressions of FN and CL in Tongguan Capsule group and finateride group were significantly lower than than those of placebo group （P〈0.01）, whereas the expressions of MT1-MMP and MMP-2/TIMP-2 increased obviously （P〈0.01）. Conclusion ECM depositing in prostate tissue is the major pathohistological characteristics of BPH. Tongguan Capsule had obvious efficiency for treatment of BPH in the study, which might be asssociated with regulating the composition of ECM and expressions of related regulatory factors, and further prevention of ECM depositing.