运用吖啶橙试验对302例不育症患者精子DNA完整性的评估

Evaluation of sperm DNA integrality of 302 patients with infertility using acridine orange staining technique

目的探讨吖啶橙试验在精子DNA完整性评估中的应用价值。方法选择男性不育症患者302例。通过吖啶橙试验(AOT)与计算机辅助精液分析(CASA)检测DFI(精子DNA损伤指数,即精液标本中存在DNA损伤的精子所占的比率)和精液常规参数。结果所有302例不育患者中,DFI异常者72例,占23.84%。共260例患者精液常规参数异常,其中DFI异常50例,占19.23%;42例患者精液常规参数正常,DFI异常22例,占52.3 8%。畸形率异常组,DFI异常率为51.06%(24/47);活动率低下组,DFI异常率为38.65%(63/163);精子密度低下组,DFI异常率为36.67%(22/60)。精子活动率和精子密度与DFI均呈负相关,r分别为-0.297,-0.217,P均<0.01;精子畸形率与DFI呈显著正相关,P<0.01,r=0.352。结论评估精子DNA完整性在不育症临床上有重要作用,吖啶橙试验能够满足这一需要,值得推荐。

Objective To evaluate the clinical value of acridine orange staining technique （AOT） for analysis of sperm DNA integrality in male infertility. Methods Semen samples of 302 male infertilite patients were analyzed in the study. Sperm DFI（DNA damage index） was detected by AOT and semen routine parameters by CASA. Results Of all 302 patients, 72 of them （23.81%） had abnormal DFI. Of all 260 patients with abnormal semen parameters, 50 of them（19.23%） had abnormal DFI.Of all 42 patients with normal semen parameters, 22 of them （52.38%） had abnormal DFI.In the group with abnormal malformation rate, motility and density, the rates of abnormal DFI were 51.06% （24/47）, 38.65%（63/163） and 36.67% （22/60） respectively. DFI was negatively correlated with sperm motility and density（r=-0.297,-0.217, P〈0.01）, positively with the malformation rate（r=0.352, P〈0.01）. Conclusion Analysis of of sperm DNA integrality was very important for diagnosis of male infertility in clinic. AO test deserved to be recommended in clinic.