摘要
目的:研究积雪草苷在大鼠体内的药动学特点。方法:用高效液相色谱法,以葫芦素B为内标,甲醇为血浆蛋白沉淀剂,水-乙腈(24∶76)为流动相,测定一次性给大鼠尾静脉注射积雪草苷(42或126mg·kg-1)后SD大鼠血浆中积雪草苷的浓度。采用3p97程序计算药动学参数。结果:积雪草苷在SD大鼠体内的药动学符合二室开放模型,2个剂量组的t1/2α分别为1.89和1.73min,t1/2β分别为27.08和26.81min,K21分别为0.24和0.20min,K10分别为0.039和0.031min,K12分别为0.11和0.13min,AUC分别为1442.11和5749.78mg·min·L-1,CL分别为0.029和0.022mL·g-1·min-1,MRT分别为39.08和38.70min。结论:积雪草苷在SD大鼠体内的分布消除较快,呈一级动力学特征。
OBJECTIVE： To investigate the pharmacokinetic characteristics of asiaticoside in rats. METHODS： HPLC method was used to determine the plasma concentration of asiaticoside in SD rats with single dose of asiaticoside （42 mg·kg^-1 or 126mg·kg^-1） via tail vein. Methanol was used to precipitate the plasma protein and water-acetonitrile （24 ： 76） was used as mobile phase. Cucurbitacin B was applied as internal standard. 3p97 program was used to calculate pharmacokinetic parameters. RESULTS： The pharmacokinetics of asiaticoside in SD rats conformed to two-compartment open model. The main pharmacokinetic parameters of two groups were as follows：t1/2α 1.89 min vs. 1.73 min;t1/2β：27.08 min vs. 26.81 min;K21： 0.24 min^-1 and 0.20 min;K10：0.039 min and 0.031 rain; K12：0.11 min and 0.13 min; AUC： 1 442.1 mg·min·L^-1 vs. 5 749.78 mg·min·L^-1;CL：0.029 mL·g·min^-1 vs, 0.022 mL·g·min^-1;MRT： 39.08 min vs. 38.70 rain, respectively. CONCLUSION： The pharmacokinetics of asiaticoside show a rapid distribution and elimination process in SD rats and is of first-order dynamic characteristics.
出处
《中国药房》
CAS
CSCD
北大核心
2010年第35期3270-3272,共3页
China Pharmacy
基金
留学回国人员科研启动基金(2006-331)
