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异戊烯焦磷酸对慢性乙型肝炎患者外周血γδT细胞增殖及干扰素γ分泌的影响 被引量：2

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摘要 目的:观察异戊烯焦磷酸刺激慢性乙型肝炎(CHB)患者外周血单个核细胞(peripheral bloodmononuclear cells,PBMCs)体外培养后,其中的γδT细胞增殖及IFNγ分泌情况。方法:体外用异戊烯焦磷酸刺激PBMCs培养10d,计数培养前后细胞总数并采用流式细胞术检测γδT细胞含量;收集第1、4、7、10天上清,ELISA法测定上清中IFNγ的含量。结果:正常组和CHB患者组PBMCs总数都有明显增殖,增殖的细胞主要为γδT细胞,正常组和CHB组分别达(55.65±6.88)%和(54.08±4.28)%,均能有效分泌IFNγ,在第7天达分泌高峰,但患者组分泌峰值低于正常组。结论:异戊烯焦磷酸能有效诱导CHB患者外周血γδT细胞增殖,并且有效分泌IFNγ。
作者 桂万羊 马勇 贺潇
机构地区 安徽省铜陵市有色职工总医院 解放军第
出处 《实用医学杂志》 CAS 北大核心 2010年第17期3087-3089,共3页 The Journal of Practical Medicine
关键词 肝炎 乙型 慢性 异戊烯焦磷酸 干扰素Γ ΓΔT细胞
分类号 R512.62 [医药卫生—内科学]
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参考文献11

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共引文献73

同被引文献35

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实用医学杂志
2010年 第17期

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