模拟不同海拔高住低练对大鼠腓肠肌热休克蛋白70的影响

Effects of HSP70 on Rats Gastrocnemius Muscle in Hypoxia Training

目的:观察模拟不同海拔高住低练的应激对大鼠红腓肠肌保护性蛋白热休克蛋白70的影响。方法:将60只SD大鼠按体重随即分为6组,高住低练模型是采用在14.5%和12.7%两种低氧浓度模拟高原居住然后常氧以35 m/min×30 min(每3天递增5 min)训练模型,共4周。取大鼠红腓肠肌进行RT-PCR技术和免疫组织化学技术检测HSP70的表达。结果:单纯训练、低氧暴露和低氧训练均能诱导HSP70表达;与单纯训练组比较,单纯低氧暴露和低氧训练组的HSP70表达增加(P<0.05),其中低氧14.5%和低氧训练12.7%两组表达增加显著(P<0.01)。结论:低氧和训练或低氧训练均诱导热休克蛋白70表达增加,低氧训练对热休克蛋白70表达影响比单纯低氧暴露或训练的刺激方式更加明显。

Objective： To observe the expression of the rats gastrocnemius protective protein and heat shock protein 70 under the simulated hypoxia training at different altitudes,and provided hypoxia training of study foundation.Method： Sixty healthy adult male SD rats were randomly divided into six groups according to their body mass.Adopting the oxygen content of 14.5% and 12.7% hypoxia models of ‘living high-training low’,increase the exercise intensity gradually for 4 weeks.And after that,take the rat’s gastrocnemius to test the expression of HSP70 with RT-PCR and immunohistochemistry technologies.Results： The expression of HSP70 was all able to be inducted from normal training,hypoxia exposure and hypoxia training;compared with normal training,the expression of HSP70 of hypoxia training groups increased（P0.05）under the hypoxia condition,and the expression of Group LH14.5%O2 and Group Hilo12.7 %O2 increased significantly（P0.01）.Conclusion： Hypoxia and training,or hypoxia training can increased the expression of HSP70,the impact of the stimulation of hypoxia training to the expression of HSP70 increased compared with hypoxia exposure.